The experiment consists of two separate components:
YING-B1 with liquid cultures only - the samples were accomodated in boxes 2,3,4,5
YING-B2 with solid surface culture only - the samples were accomodated in triangle 1,2
see attachment: YING hardware
These experiment sub-units will be performed in separate experiment hardware and have different scientific requirements.
Requirements on telemetry / data downlink / storage:
• Temperature data: 10 minute time resolution, 0.5°C; data to be returned with samples. Measurements from hand-over to hand-over.
• Passive ionizing radiation measurement (High LET & Low LET radiation dosimeters)
• Time of experiment activities to be recorded; facility data to be downlinked after completion of mission (within a few weeks of end of mission preferable)
- If possible, there shall be two photo-sessions during the active phase of the experiment. This is an “optional” requirement that would benefit to the scientific outcome of the experiment.
- Two sessions of photography in-flight, preferably FD4 & FD8 (+/-1 day).
- Field of view shall include the entire culture plate, with at least half of the samples visible.
- At least half of each culture vessel must be visible.
- Photograph must be focussed on culture surface i.e. yeast colony, the size of each yeast colony should be visible.
- Resolution of photos should be in millimetre range, reflections are to be minimised.
- Two photo-sessions for each container is the optimal situation. Two photo-sessions for two containers is the second-best option. Two sessions for only one container is still better than no photo-sessions at all.
- In order for the colonies to be observable for photography, the culture chamber shall not allow condensation to build-up.
EXECUTION OF EXPERIMENT
The experiment was carried to the ISS on the Soyuz 20S mission, which launched on 30 September 2009. The experiment used two configurations, one in liquid culture and one with solid substrate. The 26 experiment containers were processed between 2 and 7 October 2009, with only 2 of 26 cultivation chambers failing. As part of the sample processing and fixation, automated filtration and fixation of the cultivation chamber was successfully commanded from BIOLAB. After termination of the processing the experiment containers were stored at +4 degrees Celsius in the Thermal Control Unit of Biolab and returned with flight 18S.
• Record of time of experiment steps (automatically recorded)
• Temperature profile
• Photo’s (detailed resolution requirements to be defined.
• RNAlater fixed Saccharomyces cerevisiae samples on solid substrate subject to experimental protocol
• Passive radiation detectors (low LET & high LET range) exposed to flight environment in close proximity to experiment samples
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