MATISS-2 - Microbial Aerosol Tethering on Innovative Surfaces in the international space Station (CNES National Contribution)
  1. 2018 • ISS Increments 55-56
  2. 2018 • ISS Increments 57-58
  3. 2019 • ISS Increments 59-60
Life Sciences:
  • Biology
L. Lemelle (1), C. Place (1), C. Vaillant (1), J.F. Palierne (1), D. Le Tourneau (1), G. Nonglaton (2), P. Marcoux (2), J.C. Baritaux (2), V. Rebuffel (2), J. Teisseire (3), E. Garre (3), P. Benarroche (4), L. Campagnolo (4)
ENS - Ecole Normale Supérieure de Lyon
Laboratoire Joliot Curie - Laboratoire de Physique
46 allée d’Italie
69002 Lyon
CEA - French Alternative Energies and Atomic Energy Commission
17 avenue des Martyrs
38054 Grenoble
Les Miroirs
18, avenue d´Alsace
92400 Courbevoie
Biocontamination in manned spacecrafts and in future habitats could have significant impacts on crew health and biodegradation of equipment. Although there are no reported microbiological events onboard that had a critical impact on the crew, multiple reported contamination events indicate that the current prevention, monitoring and mitigation methods have to be optimized and new methods need to be considered.

In particular, there is a need for decreasing the dependency of crew action for cleaning actions, while being more efficient and reliable. The current cleaning strategy consists in manually wiping the contaminated surface with disinfectants. In addition of being time consuming and laborious, this method requires the use of chemicals that can release toxic particles in the air. Furthermore, it has been demonstrated that microorganisms can develop resistance to the used disinfectants. Finally, an accumulation of biocontamination has been observed in hardly accessible areas, such as behind the racks or next to the pumps and water pipes. To reach and wipe these areas with good efficiency can be complicated. In this context, anti-microbial surfaces that inhibit or reduce the ability of microorganisms to grow on the surfaces are of high interest.

MATISS experiments aims to demonstrate that surfaces with hydrophobic properties could be a possible answer applicable on the scale of spacecraft. The hydrophobic covers are indeed already implemented in numerous industrial fields (aeronautics, housing environment, textile, optometry, automobile, medical). By reducing the contact area of the droplets of water with surfaces, the hydrophobicity allows at the first order to limit the fraction of surface which is contaminated. It also limits the adhesion of microorganisms to surfaces.

Justification for Need of Space Experiment
Antimicrobial surfaces are currently used on Earth in a number of sectors, such as healthcare, food industry, water industry, textiles. Consequently, numerous tests and standards exist for evaluating antimicrobial surfaces for terrestrial applications. However, they are not being adequate for closed habitats environment, and do not take into account parameters specific to manned spaceflights such as environmental conditions including radiation effects and impact of microgravity on the attachment mechanisms of contaminants on modified surfaces.

In the context of future manned mission scenarios of longer duration, higher isolation and the utilization of an increasing number of closed loop life support systems, the coating or surface modification must be effective ‘in-use’.

Studies are currently being conducted to derive standard requirements for the evaluation of antimicrobial surfaces in the very specific context of manned spacecraft, but the complexity of the environment need to be derived in precise test parameters. In particular, the concentration and content of test inoculum lead to complex and hardly reproducible tests conditions, calling for in-situ data collection to support theoretical studies.

MATISS set of experiments (MATISS-1 and MATISS-2) aims to provide the minimum prerequired data necessary to support the development of antimicrobial surfaces for space applications and to initiate a long-term R*D project to develop and produce industrially an innovative process of surface treatment of the materials suitable for future spacecraft.

In this context, the technological demonstration MATISS-1 (Microbial Aerosol Tethering on Innovative Surfaces in the international Space Station-1) was evaluated during PROXIMA Mission by the European Astronaut Thomas Pesquet (Inc 50/51).

The objective of MATISS-1 consisted in evaluating the general operational concept and to validate the design of the MATISS payload, which protects the glass surfaces while sampling ISS atmosphere. In addition, MATISS-1 provided baseline data points of a set of variables (type of surfaces, location, duration of exposure).

MATISS-2, is the continuation of MATISS-1, and aims to establish the kinetics of the development of the contamination on the hydrophobic/control surfaces.

MATISS-1 evaluated four identical sample holders loaded with 5 different superhydrophobic coatings and one control applied on one substrate (glass). The sample holders were exposed at 3 different locations (EPM rack, EDR rack and RGSH) during a fixed duration (6 months).

In this respect, the characterization of the deposits formed on the surfaces from MATISS-1 (return in the laboratory in June 2017) will establish a reference of the level and the type (dusts, biomovies) of contamination of the surfaces for a 6 months of exposure in three different zones.

MATISS-2 aims at testing four identical sample holders loaded with at least 3 types of surfaces (two hydrophobic surfaces and a control) possibly over two materials (glass and metal). The two hydrophobic surfaces will be selected among the five types tested during MATISS-1, based on the results observed in MATISS-1. MATISS-2 sample holders will be installed at the same time in a single location, location chosen among the three evaluated by MATISS-1. The duration of exposure of each sample holder is kept variable. In other words, the results of MATISS-1 will indicate the duration of exposure of the first sample holder of MATISS-2. The results of the first MATISS-2 sample holder downloaded will indicate the duration of exposure of the second sample holder of MATISS-2 etc.

An important specificity of MATISS-2 is that the duration of exposure will be optimized with the results of the surface’s analyses done on-ground in a dynamic operational mode. Each sample holder downloaded will provide information that impact the duration of exposure of the remaining sample holders on-board.

The interdependence between MATISS-1 and MATISS-2 is summarised as follows:

Superhydrophobic coating
MATISS-1 (tech demo): 5
MATISS-2: 2 or 3 

MATISS-1 (tech demo): glass
MATISS-2: glass and metal

Location of exposure
MATISS-1 (tech demo): 3 locations
MATISS-2: 1 location

Duration of exposure
MATISS-1 (tech demo): 6 months
MATISS-2: Varying

Previous Flight Experiments:
MATISS-1 - Microbial Aerosol Tethering on Innovative Surfaces in the international space Station (CNES)
ISS Increments 49-50 / 51-52 - 2016-217

This technology demonstration was sponsored by CNES and was performed during the "Proxima" mission of Thomas Pesquet (November 2016-June 2017)
Short summary of MATISS-1 

MATISS-2.5 - Microbial Aerosol Tethering on Innovative Surfaces in the international space Station
ISS Increments 61-62 - 2019 
The Matiss Sample Holders were installed on 23 August 2018. The samples were exposed for different periods of time and successively brought back to Earth on different cargo and crewed crafts. 
The last sample returned with the SpX-18 mission. 

Following unberthing from the International Space Station (ISS) and release from the Space Station Remote Manipulator System (SSRMS) at 14:59 UTC on 27 August 2019, the SpaceX’s CRS-18 Dragon spacecraft splashed-down in the Pacific Ocean around 20:20 UTC.

Dragon CRS-18 brought back to Earth the following European experiment samples:
- deep-frozen samples of the Amyloid Aggregation experiment;
- deep-frozen microbes of the BioRock experiment;
- algae of German Aerospace Center DLR’s PhotoBioreactor;
- Matiss-2 experiment.

Operational Overview
The experiment consists of the following activities:
- MATISS Installation: The sample holders are installed in one pre-determined locations in Columbus; The protective Kapton Tape being removed to allow air flow circulation and consist in the start of the exposure time
- MATISS Exposure: The sample holders are exposed unattented in the cabin for various durations.
- MATISS Deinstallation: The sample holders are removed one by one from their location, and protected with Kapton Tape before download
- MATISS holder surfaces will be analysed on ground to determine the remaining holders exposure duration.

MATISS Sample Holders
MATISS-2 uses MATISS-1 sample holders already certified for flight. 
The purpose of MATISS Sample Holders is to contain and protect the hydrophobic surfaces, which are the core of the MATISS-2 Experiment.
The sample Holder contains 1 witness surface and 5 “transformed surfaces” which have the same dimensions (glass 22 mm x 22 mm x 170 μm).
A schematic of the Sample Holder without the system of protecting lids and drawers is shown in Figure 1

MATISS Sample Holder (FM) is depicted in Figure 2.

The surfaces are mounted in the Sample Holder, which technical drawings can be found in Figure 3 and 4

Base: The base is made of Aluminium Au4G (2017A), is 2mm high and acts as the structure of the Sample Holder
Plate: The Plate is made of Viton (60C7), is 1mm high and is meant to avoid direct contact between glass/Aluminium, and to absorb vibrations during launch and return.
Kapton Tape: The Kapton Tape (0.08mm) is glued at the back of the surfaces, to partially retain glass fragments in case of glass damage.
Seal: The seals are made of Kapton Tape to absorb vibrations during launch and return.
Grid: The Grid is made of Aluminium Au4G (2017A), is 5 mm high and protects the surfaces from impact of small objects by reducing the exposed surfaces.
Lid: The Lid is made of Polycarbonate and its height is 5mm.The purpose of the lid is to prevent tool impact, inadvertent kick or bump during exposition in the station.
The Sample Holder is kept with Inox A2 screws (ISO 10642 / DIN 7991) M3x10, M3x8 and M3x6.

Scheduling Constraints
- All MATISS-2 sample holders shall be installed at the same time in a single pre-defined location.
- All MATISS-2 sample holders shall be downloaded as soon as possible pending flight opportunity after the exposure time completion (known for the first sample holder, unsettled for the three remaining sample holders)

Parameters Measured
On board: cabin temperature and humidity. 

The analyses are performed on ground with the download of the four sample holders.

Two campaigns of analyses are foreseen:
The first one will be carried out exclusively with non-destructive analyses in order to bring as fast as possible a general overview of the deposits on the lamella. Three types of techniques will be possibly coupled to get such overview:
- optical microscopy inspection
- AFM microscopy
- Optical forward-scattering
The second campaign will be carried out with high resolution and possibly with destructive techniques on few selected samples. The following four types of techniques are considered:
- optical microscopy inspection
- Raman spectroscopy
- Bacterial culture
- Environmental microscopy
Preliminary Results
All the sample holders were retrieved and received in excellent mechanical and chemical preservation states. The science team is performing first observations and analysis.

Expected Results and Hypothesis
The expected result is that hydrophobic surface will limit the amount of surface covered by contamination and in particular by biocontamination.

The hypothesis that is done in this project is that such effect will be persistent but visible on long-term exposure.
L. Lemelle, M. Salomé, M. Fialin, A. Simionovici, P. Gillet, (2004), "In situ detection and Xray imaging of microorganisms distribution on the Tatahouine meteorite", Spectrochimica Acta Part B: Atomic Spectroscopy, 59B, pp. 1703-1710.
L. Lemelle, A. Simionovici, M. Salomé, P. Bleuet, J. Susini, P. Gillet, (2007), "In situ search for life traces in extraterrestrial samples by synchrotron x-ray fluorescence 2D and 3D imaging", Proceedings of SPIE - The International Society for Optical Engineering, 6694, 1-8, DOI: 10.1117/12.733167, pp. 669414.
L. Lemelle, J.F. Palierne, E. Chatre, C. Place, (2010), "Counter-clockwise circular motion of bacteria swimming at air/liquid interface", Journal of Bacteriology, 192, 23, DOI: 10.1128/JB.00397-10, pp. 6307-6308.
A. Berrier, M.C. Schaafsma, G. Nonglaton, J. Bergquist, J. Gómez, (2012), "Selective detection of bacterial layers with terahertz plasmonic antennas", Biomedical Optics Express, 3, 11, DOI: 10.1364/BOE.3.002937, pp. 2937-2949.
L. Lemelle, J.F. Palierne, E. Chatre, C. Vaillant, C. Place, (2013), "Curvature reversal of the circular motion of bacteria swimming at solid/liquid interface", Soft Matter, 41, pp. 9759-9762.
P.R. Marcoux, M. Dupoy, A. Cuer, J.L. Kodja, A. Lefebvre, F. Licari, R. Louvet, A. Narassiguin, F. Mallard, (2014), "Optical forward-scattering for identification of bacteria within microcolonies", Applied Microbiology and Biotechnology, 98, 5, DOI: 10.1007/s00253-013-5495-4, pp. 2243-2254.
J.C. Baritaux, A.C. Simon, E. Schultz, C. Emain, P. Laurent, J.M. Dinten, (2016), "A study on identification of bacteria in environmental samples using single-cell Raman spectroscopy: feasibility and reference libraries", Environmental Science and Pollution Research, 23, 9, DOI: 10.1007/s11356-015-5953-x, pp. 8184-8191.
click on items to display

Figure 1: MATISS surfaces.

Figure 2: MATISS Sample Holder.

Figure 3: Sample Holder - exploded view.

Figure 4: MATISS Schematic.

Figure 5: Everybody knows a clean house is a healthy place to live, but what if you live on the International Space Station? Air and water are constantly recycled and waste can only be removed when a spacecraft departs for Earth every few months. For the six astronauts living in humanity’s habitat in space, keeping the Station clean is an important part of their life to avoid bacteria and fungus. Every Saturday is cleaning day, when the whole crew wipe surfaces, vacuum and collect waste. The Matiss experiment is investigating antibacterial properties of materials in space to see if future spacecraft could be made easier to clean. The experiment consists of plaques placed in the European Columbus laboratory and leave for at least three months. France’s CNES space agency, in collaboration with the ENS universityof Lyon, research institute CEA Tech-LETI and construction company Saint-Gobain, selected five advanced materials that could stop bacteria from settling and growing on the surface. A sixth element, made of glass, is used as control material. The materials are a diverse mix of advanced technology - from self-assembly monolayers and green polymers to ceramic polymers and water-repellent hybrid silica. The smart materials should stop bacteria from sticking to the surface and growing, effectively making them easier to clean and more hygienic - but which one works best? photo credit: NASA

Figure 6: The Matiss-2 experiment was placed in Europe’s space laboratory Columbus for just under a year collecting dust and bacteria. Researchers will now analyse the surfaces to see which materials are least hospitable to unwanted bacteria – focusing on materials that expel water. Copyright: ESA/NASA
© 2023 European Space Agency